Protocollen
KineticPep does not publish dosing protocols. This page explains why, and covers the laboratory-workflow guidance we do provide — reconstitution, aliquoting, storage, and record-keeping — so researchers can handle our products safely and reproducibly.
Why we do not publish human or animal protocols
KineticPep products are sold strictly for laboratory research use only. Publishing dosing tables, cycle guides, or human-use protocols would misrepresent the product as intended for a clinical or performance context and violate the terms under which we operate. If your work involves a living organism, a licensed physician or veterinarian is the correct source for protocol design. If your work is in-vitro, protocol parameters (concentration, incubation time, buffer conditions) depend entirely on your assay and are not something a supplier can prescribe.
Reconstitution — general workflow
The steps below are the standard laboratory workflow for reconstituting a lyophilized peptide vial.
1. Choose the correct carrier
Most peptides reconstitute in Bacteriostatic Water (0.9% benzyl alcohol). Acid-sensitive peptides such as BPC-157, TB-500, and copper-complex peptides (GHK-Cu) require Acetic Acid Water 0.6% instead. Using the wrong carrier can cause visible precipitation, cloudiness, or silent chemical degradation. Both solvents are stocked in our catalogue as separate SKUs.
2. Bring both vials to room temperature
Take the lyophilized vial and the carrier vial out of the refrigerator and let them stand at room temperature for 15–20 minutes. Cold solvent hitting a cold lyophilizate produces a slow, uneven dissolution.
3. Draw the carrier
Use a sterile syringe with the finest gauge that will still draw the volume without cavitation (typically 27–30 G for volumes of 1–5 ml). Wipe the carrier vial rubber stopper with an alcohol swab, invert the vial, and withdraw the calculated volume.
4. Inject the carrier down the wall of the peptide vial
Insert the needle at an angle and let the carrier run gently down the inside wall of the vial onto the lyophilizate, rather than jetting directly onto the powder. This preserves the peptide from mechanical stress and foaming.
5. Swirl, do not shake
Rotate the vial gently between your fingers until the lyophilizate is fully dissolved. Do not vortex or shake vigorously; peptides are surfactant-active and shaking generates foam that traps material at the air–liquid interface, reducing effective concentration.
6. Inspect and store
The reconstituted solution should be clear and colourless (GHK-Cu is a pale blue exception due to the copper complex). Any cloudiness, particulates, or discolouration indicates that the peptide is compromised. Store the reconstituted vial upright at 2–8 °C, shielded from light. Use within approximately 4 weeks depending on the specific peptide.
Aliquoting for long-term storage
For experiments spanning longer than four weeks, aliquot the reconstituted peptide into sterile microcentrifuge tubes (single-use volumes), snap-freeze in liquid nitrogen or a −80 °C freezer, and thaw individual aliquots as needed. Repeated freeze–thaw cycles degrade most peptides.
Record-keeping
Best practice is to note on every vial and aliquot: peptide name, batch number (from the KineticPep COA), reconstitution date, carrier and volume used, resulting concentration in mg/ml, and expiry (reconstitution date + 4 weeks). A simple lab notebook entry cross-referencing the KineticPep batch to your internal sample ID makes retrospective analysis straightforward.
Certificate of Analysis for every batch
The COA for the specific batch you receive documents the actual HPLC purity, mass-spectrometry identity, net content, and appearance for that lot. Request it from [email protected] and keep the PDF filed alongside your experimental records.
Where to go from here
See the Kwaliteit page for details on how we test each batch, the Wetenschap page for background on the peptide families we stock, and the FAQ for the fifteen questions researchers ask most often before ordering.
